Primary Skeletal Muscle Cell Growth Kit contains components that when added to Mesenchymal Stem Cell Basal Medium (ATCC PCS-500-030) create a complete ATCC Primary Cell Solutions culture environment for skeletal muscle cells derived from a normal human skeletal tissue (eg, Primary Skeletal Muscle Cells, Normal, Human, ATCC PCS-950-010). The medium formulation is designed to support normal skeletal cell proliferation and growth. No feeder layers, extracellular matrix proteins, or other substrates are required.